This Short Communication published in the European Journal of Neuroscience back in 2000 by Lucia Galli-Resta, Elena Novelli, Maila Volpini and Enrica Strettoi was a paper I did not know existed. That said, I ran into it the other day looking for some reference material and found it to be quite useful. This communication represents an analysis of the cholinergic amacrine cell mosaics in the C57Bl6/J murine retina. It served as a useful baseline for cell positioning, and cell mosaicing in both cholinergic arrays of the retina and is a nice analysis that should serve as a reference point for future genetic analysis studies in normal and pathological retinal tissues. Enrica Strettoi’s laboratory has been carefully exploring the functional organization of the retina for some time now in the normal and pathological states and its always a joy to discover her work in the literature, even if it is 13 years old.