Interesting paper: Characterization Of Neurite Outgrowth And Ectopic Synaptogenesis In Response To Photoreceptor Dysfunction

Authors Stylianos Michalakis, Karin Schäferhoff, Isabella Spiwoks-Becker, Nawal Zabouri, Susanne Koch, Fred Koch, Michael Bonin, Martin Biel, and Silke Haverkamp have a new paper out that looks at the earliest gene microarray analysis results associated with neurite outgrowth in the degenerate retina.  The title is a overly broad, but the results focusing on gene expression changes in the A3B1 mouse retina (a CNGA3/CNGB1 double-knockout) are intriguing, particularly their proposal that Tp53, Smad and Stat3 signaling contribute to synaptic plasticity at least.


In the mammalian retina, light signals generated in photoreceptors are passed to bipolar and horizontal cells via synaptic contacts. In various pathological conditions, these second-order neurons extend neurites into the outer nuclear layer (ONL). However, the molecular events associated with this neurite outgrowth are not known. Here, we characterized the morphological synaptic changes in the CNGA3/CNGB1 double-knockout (A3B1) mouse, a model of retinitis pigmentosa. In these mice, horizontal cells looked normal until postnatal day (p) 11, but started growing neurites into the ONL 1 day later. At p28, the number of sprouting processes decreased, but the remaining sprouts developed synapse-like contacts at rod cell bodies, with an ultrastructural appearance reminiscent of ribbon synapses. Hence, neurite outgrowth and ectopic synaptogenesis in the A3B1 retina were precisely timed events starting at p12 and p28, respectively. We therefore performed microarray analysis of retinal gene expression in A3B1 and wild-type mice at those ages to evaluate the genomic response underlying these two events. This analysis identified 163 differentially regulated genes in the A3B1 retina related to neurite outgrowth or plasticity of synapses. The global changes in gene expression in the A3B1 retina were consistent with activation of signaling pathways related to Tp53, Smad, and Stat3. Moreover, key molecules of these signaling pathways could be localized at or in close proximity to outgrowing neurites. We therefore propose that Tp53, Smad, and Stat3 signaling pathways contribute to the synaptic plasticity in the A3B1 retina.


Image modified from supplemental material 6 from the authors paper.

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