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Silver Intensification for Immunocytochemistry


    Procedure
  1. Prepare silver intensification solutions. Stock solution C may be prepared in advance. Prepare stock solutions A and B separately prior to intensification and use within 1 hour.
  2. Use the working solution immediately as it lasts only 10 min. Add 25-50 microliters of solution per well in quick succession; a small disposable dropper works best. (Alternative: make 15 ml of working solution and process in a plastic slide cassette). Expose sections to the solution for 4-8 minutes in a dark location, such as under an aluminum cover. You needn't work in absolute darkness, but the background decreases slightly if the intensification occurs away from bright light. The reaction is temperature sensitive and slows substantially below 24°C.
  3. Stop the reaction with a brief dip in 5% acetic acid.
  4. Wash for 10 minutes in deionized water and dry with an air canister.
  5. Cover slip in a medium of your choice.




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© 2000 Robert E. Marc
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