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METHODS: Standard Plastic Embedding Protocol

Stock Solutions:
0.1 M phosphate buffer, pH 7.4 (PB)
100% Acetone
100% anhydrous MeOH and 75%, 85%, 95% MeOH in dH2O
DMP30
Eponate resin
DDSA

Mixing Active Resin: Mix resin fresh or thaw from freezer. Work quickly to prevent components from hydrating. Wear gloves as these components may cause contact dermatitis and the synthesis of certain components involves potential carcinogens. Prepare under a hood. Dehydration and Infiltration Schedule: All volumes 5-10 ml unless noted otherwise. This procedure is designed for tissue samples < 1 mm thickness, presumed to be in fixative. Disclaimer: As conditions of use are outside our control, we make no warranties, express or implied, and assume no liability in connection with use of this protocol.

© 2000 Robert E. Marc
This protocol may be copied and distributed for educational and non-profit
purposes as long as acknowledgement of our copyright is included
in every instance of use. It may not be sold or distributed for commercial gain.


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