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    Procedure
  1. Working solution: Dilute stock Na methoxide/ethoxide 1:4 v/v in anhydrous EtOH. It is critical that all solutions be absolutely anhydrous. If you doubt the 100% EtOH, don't use it.
  2. Place slides into glass or steel carriers (not plastic) and immerse in the working solution. The general etching time is 14 min/micron and is linear (e.g. 30 min for 2 microns, 7 min for 500 nm, 1 min for gold 90 nm sections). Note that the working solution can be used for several days if kept anhydrous.
  3. Drain the excess solution by blotting the carrier onto a stack of paper towels.
  4. Immediately immerse in 100% EtOH or MeOH and wash 2 min. Repeat 3X with fresh alcohols.
  5. Rinse slides 5 min in running cool tap water
  6. Dip slides in dH2O and air dry.
Disclaimer: As conditions of use are outside our control, we make no warranties, express or implied, and assume no liability in connection with use of this protocol.

© 2000 Robert E. Marc
This protocol may be copied and distributed for educational and non-profit
purposes as long as acknowledgement of our copyright is included
in every instance of use. It may not be sold or distributed for commercial gain.

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