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Shannon Odelberg Lab
Paraformaldehyde-Lysine-Periodate fixative

notes modified by REM underlined

    PLP fixative
  1. Add 0.05g paraformaldehyde to 75 ml dH2O in a 100 glass beaker, with a small spinbar and heat on stir plate in Hood # 1. (next to the Freezer).
  2. When it reaches 65°C or begins to bubble add 2-3 drops 1M NaOH and wait until the solution clears. Usually less than 30s.
  3. Add 20 ml 5X buffer and adjust final volume to 100 ml with dH2O. Aliquot and freeze at -20°C.
    5X Buffer 200 ml
  • 10 mM sodium periodate = 2.14g
  • 100 mM lysine-HCl= 18.27g
  • 150 mM NaCl= 1.74g (adjusted for mammals)
  • 3 mM KCl=12 ml of 0.25M stock
  • 10 μM CaCl2 100 μl of 100mM stock
  • 5 mM HEPES = 1.19g
  • Check and adjust pH to 7.4

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