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New Manuscripts
∞ Go to the new manuscript page
∞Read the annotated Science reviews: 2009.10.06
∞ Go to JNM Viking manuscript page
Parameters for Rabbit Retinal Connectome
∞ Launch Fig 1S small array (800 pixels wide)
∞ Launch Fig 1S large array (4000 pixels wide)
Retinal Connectome RC1 Quicktime ® movies (large files)
↓ DOWNLOAD ... a colorful 3D retinal volume rendering (122 Mb)
↓ DOWNLOAD ... a trip down a bipolar cell axon (35 Mb)
↓ DOWNLOAD ... and page through the entire TEM volume at 1K2 (225 Mb)
Cell numbers for RC1
284 BCs
140 EBCs
124 GBCs
011 RBCs
003 BBCs
279 ACs
167 YACs
009 on sb acs
118 GACs
009 Aii ACs
005 Ai ACs
019 HCs
130 MG
023 UG
018 GCs
Basic histologic parameters
Field diameter size = 250 μm
Nominal section thickness 90 nm (ideal 70 nm)
Stack thickness 35 μm (GCL - IPL - ACL)
Stack sections = 371
TEM magnification 5000x, 2.18 nm/pixel
TEM tile size 8.7 x 8.7 μm
Tiles/mosaic 900-1100 (15% overlap)
Mosaic acquisition time 5 h
Volume acquisition time 133 d
Volume build time 33 d
Raw volume size 16 Tb
Display volume + 2 backups size: 50 Tb
Definitions
A Canonical field is a region containing no less than
three copies of the rarest cell class. This is our ideal volume.
A Canonical tile is a region containing the rarest cell
class and its Voronoi domain.
Synaptic sampling parameters
| Sampling Area (mm2) | 0.04 mm2 | 0.01 mm2 |
| BC density | 40000/mm2 | 40000/mm2 |
| BCs sampled | 1600 | 400 |
| Ribbons/BC | 15 | 15 |
| Ribbon synapses | 24000 | 600 |
| Conventional syn/ribbon | 2 | 2 |
| Total synapses | 72000 | 36000 |
| Synapses scored/hr | 10 | 10 |
| Total workdays | 900 | 225 |
| Total work years | 3 | 0.75 |
Population sampling parameters
| Sampling Area (mm2) | 0.04 mm2 | 0.01 mm2 |
| BCs sampled | 1600 | 400 |
| ACs sampled | 800 | 200 |
| GCs sampled | 200 | 50 |
| dACs sampled | 100 | 25 |
GC sampling parameters
Class
Percent
Copies 0.04
Copies 0.01
1a
2.8
6
1-2
1b
6
12
3
1c
5.7
11
2-3
2
4.2
8
2
3
12.2
24
6
4
7.7
15
3-4
5
7
14
6
15
30
7
7
5.6
11
3-4
8
12.2
24
6
9
1.4
3
1
10
8.4
17
4
11
6.6
13
3
12
5.2
10
2-3
total GCs
200
50
sACs
32.7
98
27
other dACs
3.9
12
3
Total dACs
3.9
110
30
Total Neurons
310
80
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