University of Utah              The JOHN A. MORAN EYE CENTER

HOME TOOLS SCIENCE PUBLICATIONS CYTOSOMICS & METABOLOMICS



New taurine jpg for ARVO 2007
RAW P23H IMAGE CAPTURES: Each tiff is an 8-bit differential intereference image of 90 um thick resin sections through the central retina of all coded samples. Each image was captured using Nomarski optics (Zeiss 40x planapochromatic) by a Dage-MTI camera at 221 nm/pixel resolution. The images are unaltered from capture. Filename = sample code #.
  1. Camera_01.tif: right-click or ctrl-click and Save As ...
  2. Camera_02.tif: right-click or ctrl-click and Save As ...
  3. Camera_03.tif: right-click or ctrl-click and Save As ...
  4. Camera_04.tif: right-click or ctrl-click and Save As ...
  5. Camera_05.tif: right-click or ctrl-click and Save As ...
  6. Camera_06.tif: right-click or ctrl-click and Save As ...
  7. Camera_07.tif: right-click or ctrl-click and Save As ...
  8. Camera_08.tif: right-click or ctrl-click and Save As ...
  9. Camera_09.tif: right-click or ctrl-click and Save As ...
  10. Camera_10.tif: right-click or ctrl-click and Save As ...
  11. Camera_11.tif: right-click or ctrl-click and Save As ...
  12. Camera_12.tif: right-click or ctrl-click and Save As ...
  13. Camera_13.tif: right-click or ctrl-click and Save As ...
  14. Camera_14.tif: right-click or ctrl-click and Save As ...
  15. Camera_15.tif: right-click or ctrl-click and Save As ...
  16. Camera_16.tif: right-click or ctrl-click and Save As ...
  17. Camera_17.tif: right-click or ctrl-click and Save As ...
  18. Camera_18.tif: right-click or ctrl-click and Save As ...
  19. Camera_19.tif: right-click or ctrl-click and Save As ...
  20. Camera_20.tif: right-click or ctrl-click and Save As ...
  21. Camera_21.tif: right-click or ctrl-click and Save As ...
  22. Camera_22.tif: right-click or ctrl-click and Save As ...
  23. Camera_23.tif: right-click or ctrl-click and Save As ...
  24. Camera_24.tif: right-click or ctrl-click and Save As ...
  25. Camera_25.tif: right-click or ctrl-click and Save As ...
  26. Camera_26.tif: right-click or ctrl-click and Save As ...
Data sets and notes for all samples. Includes measurements of layer thickness as an Excel spreadsheet, histograms and scatterplots, and raw notes.
We measured three key parameters:
(1) The distance from the external limiting membrane (ELM) to the border of the IPL and GCL: inner + ONL
(2) The thickness of the outer nuclear layer (ONL)

....as an intermediate value, inner retina (from the OPL to the border of the IPL and GCL) = (1)-(2)

(3) The distance between the ELM and RPE - as an estimate of the outer segment layer thickness (OSL)

Because the sections are thick and may have a small tilt, the most robust values are OSL and ONL normalized by the thickness of the inner retina: ONL/inner, OSL/inner. All the data are on the hetling_bogdanovich_p23h.xls spreadsheet (right-click or ctrl-click and Save As ...). Plotting histograms of the latter show a clear multimodal pattern.

Small jpg version: histogram.jpg: right-click or ctrl-click and Save As ...
Large tif version: histogram.tif: right-click or ctrl-click and Save As ...

And plotting both these on scatterplots show what might be clusters, and I have labeled the sample numbers onto the scatterplot for convenience. The normalized onl (X axis) = onl thickness/inner retina thickness; normalized osl (Y axis) = osl thickness/inner retina thickness;

Small jpg version:het_bogdan_splot.jpg: right-click or ctrl-click and Save As ...
Large tif version:het_bogdan_splot.tif: right-click or ctrl-click and Save As ...
We have a lot of data - the SDs in any one region are less than 0.1 of the mean thickness (i.e. CV = 0.1) so I'm just giving you the means for central retina each animal for now. For example - the OSL ranges from 12 to 0 um thick - so the SDs are in the range of 1 um. Each animal has some variation - see notes below - but in general, if the ONL/OSL is thin - it is thin primarily in the central retina and tends to get thicker at the retinal margins - right at the ciliary margin in fact (growth/survival factors?). If it is thick - it is thick everywhere.

Here are the sample notes on visual examination:
Sample # Notes
1. Moderate thinning 3 nuclei thick
2. Moderate thinning 3-4 nuclei thick
3. 4-6 nuclei thick
4. Moderate thinning 3-4 nuclei thick
5. Moderate thinning 3-4 nuclei thick
6. Strong thinning 2-3 nuclei - but back up to 5 at ora
7. 2 in center - no OS - but detached - 6 at ora - retina detached in many spots
8. 5 nuclei
9. 1 nucleus layer in central retina- but up to 5-6 at ora
10. 2 nuclei thick in central retina- but up to 5 at ora
11. 3 nuclei over all retina
12. 1 over most of the retina - 2-3 at ora
13. detached - 3 over most of retina
14. 4 over most of retina
15. 4-5 over most of retina
16. 0-2 over most retina - 4-5 at ora - no OS in center
17. largely - detached 5 near ONH - 6 near ora
18. 4-5 over most retina, but spots down to 2
19. 2 over most of retina
20. 5-7 over most of retina
21. 4-5 over most of retina
22. 4 over most of retina
23. 2 over most of retina - 3-4 at ora - no OS in central
24. 3-5 over most of retina
25. 2-3 over central retina - 5-6 at ora
26. 2-3 over most retina, but short or no OS

A four panel image of codes 06, 08, 18, and 19

Caption: Differential intereference images of 90 μm thick resin sections through the central retina of four coded samples. Each image was captured using Nomarski optics (Zeiss 40x planapochromatic) by a Dage-MTI camera at 221 nm/pixel resolution. Images were max-min contrast matched and unsharp masked with a 2 pixel radius. Every sample was within 1 mm of the central pole.

The key zones of each structural layer are color coded:
  • Pink (isl + osl) = Myoid region of inner segments + outer segments
  • Green (onl) = outer nuclear layer
  • Blue (inl) = inner nuclear layer
  • Orange (ipl) = inner plexiform layer
  • Lavender (gcl) = ganglion cell layer.
Sample codes 08 and 18 show surviving onl and osl regions at about 50-80% of normal, while codes 06 and 19 lack any osl and the onl is depleted to 1-2 rows of phtotreceptor nuclei.
Small jpg image: samples_6_8_18_19_v2.jpg: right-click or ctrl-click and Save As ...
Full size 72 dpi tif image: samples_6_8_18_19_v2.tif: right-click or ctrl-click and Save As ...
Full size 72 dpi psd image with layers: samples_6_8_18_19_v2.psd: right-click or ctrl-click and Save As ...

HOME TOOLS SCIENCE PUBLICATIONS CYTOSOMICS & METABOLOMICS

Moran Eye Center 75 N Medical Dr Salt Lake City, UT 84132
University of Utah Disclaimer   ·   MarcLab Website Terms of Use/Disclaimers/Contacts