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| University of Utah The JOHN A. MORAN EYE CENTER |
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| New taurine jpg for ARVO 2007 |
| RAW P23H IMAGE CAPTURES: Each tiff is an 8-bit differential intereference image of 90 um thick resin sections through the central retina of all coded samples. Each image was captured using Nomarski optics (Zeiss 40x planapochromatic) by a Dage-MTI camera at 221 nm/pixel resolution. The images are unaltered from capture. Filename = sample code #. |
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| Data sets and notes for all samples. Includes measurements of layer thickness as an Excel spreadsheet, histograms and scatterplots, and raw notes. |
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We measured three key parameters: (1) The distance from the external limiting membrane (ELM) to the border of the IPL and GCL: inner + ONL (2) The thickness of the outer nuclear layer (ONL) ....as an intermediate value, inner retina (from the OPL to the border of the IPL and GCL) = (1)-(2) (3) The distance between the ELM and RPE - as an estimate of the outer segment layer thickness (OSL) Because the sections are thick and may have a small tilt, the most robust values are OSL and ONL normalized by the thickness of the inner retina: ONL/inner, OSL/inner. All the data are on the hetling_bogdanovich_p23h.xls spreadsheet (right-click or ctrl-click and Save As ...). Plotting histograms of the latter show a clear multimodal pattern.  Small jpg version: histogram.jpg: right-click or ctrl-click and Save As ... Large tif version: histogram.tif: right-click or ctrl-click and Save As ... And plotting both these on scatterplots show what might be clusters, and I have labeled the sample numbers onto the scatterplot for convenience. The normalized onl (X axis) = onl thickness/inner retina thickness; normalized osl (Y axis) = osl thickness/inner retina thickness;  Small jpg version:het_bogdan_splot.jpg: right-click or ctrl-click and Save As ... Large tif version:het_bogdan_splot.tif: right-click or ctrl-click and Save As ... We have a lot of data - the SDs in any one region are less than 0.1 of the mean thickness (i.e. CV = 0.1) so I'm just giving you the means for central retina each animal for now. For example - the OSL ranges from 12 to 0 um thick - so the SDs are in the range of 1 um. Each animal has some variation - see notes below - but in general, if the ONL/OSL is thin - it is thin primarily in the central retina and tends to get thicker at the retinal margins - right at the ciliary margin in fact (growth/survival factors?). If it is thick - it is thick everywhere. Here are the sample notes on visual examination: Sample # Notes 1. Moderate thinning 3 nuclei thick 2. Moderate thinning 3-4 nuclei thick 3. 4-6 nuclei thick 4. Moderate thinning 3-4 nuclei thick 5. Moderate thinning 3-4 nuclei thick 6. Strong thinning 2-3 nuclei - but back up to 5 at ora 7. 2 in center - no OS - but detached - 6 at ora - retina detached in many spots 8. 5 nuclei 9. 1 nucleus layer in central retina- but up to 5-6 at ora 10. 2 nuclei thick in central retina- but up to 5 at ora 11. 3 nuclei over all retina 12. 1 over most of the retina - 2-3 at ora 13. detached - 3 over most of retina 14. 4 over most of retina 15. 4-5 over most of retina 16. 0-2 over most retina - 4-5 at ora - no OS in center 17. largely - detached 5 near ONH - 6 near ora 18. 4-5 over most retina, but spots down to 2 19. 2 over most of retina 20. 5-7 over most of retina 21. 4-5 over most of retina 22. 4 over most of retina 23. 2 over most of retina - 3-4 at ora - no OS in central 24. 3-5 over most of retina 25. 2-3 over central retina - 5-6 at ora 26. 2-3 over most retina, but short or no OS |
| A four panel image of codes 06, 08, 18, and 19 |
 Caption: Differential intereference images of 90 μm thick resin sections through the central retina of four coded samples. Each image was captured using Nomarski optics (Zeiss 40x planapochromatic) by a Dage-MTI camera at 221 nm/pixel resolution. Images were max-min contrast matched and unsharp masked with a 2 pixel radius. Every sample was within 1 mm of the central pole. The key zones of each structural layer are color coded:
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Small jpg image: samples_6_8_18_19_v2.jpg: right-click or ctrl-click and Save As ... Full size 72 dpi tif image: samples_6_8_18_19_v2.tif: right-click or ctrl-click and Save As ... Full size 72 dpi psd image with layers: samples_6_8_18_19_v2.psd: right-click or ctrl-click and Save As ... |
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