Basic histologic parameters
Ideal section size = 0.2 mm x · 0.2 mm y · 90 nm
Stack thickness = 0.025 mm = 278 sections (ipl) ~400 GCL > OPL

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Definitions
A Canonical field is a region containing no less than
three copies of the rarest cell class. This is our ideal volume.

A Canonical tile is a region containing the rarest cell
class and its Voronoi domain.

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Basic CN sampling Frameworks

Framework	Mag	Area	Capture time (d)	current stos	stos 4x speed
-----	-----	-----	-----	-----	-----
Ideal volume	5000x	200 μm x 200 μm	35	17y	4.3y
Fast volume	3000x	200 μm x 200 μm	13	6y	1.5y
Fast small volume	3000x	100 μm x 100 μm	3	1.6	0.4y

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Synaptic sampling parameters

Sampling Area (mm2)	0.04 mm2	0.01 mm2
BC density	40000/mm2	40000/mm2
BCs sampled	1600	400
Ribbons/BC	15	15
Ribbon synapses	24000	600
Conventional syn/ribbon	2	2
Total synapses	72000	36000
Synapses scored/hr	10	10
Total workdays	900	225
Total work years	3	0.75

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Population sampling parameters

Sampling Area (mm2)	0.04 mm2	0.01 mm2
BCs sampled	1600	400
ACs sampled	800	200
GCs sampled	200	50
dACs sampled	100	25

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GC sampling parameters

Class	Percent	Copies 0.04	Copies 0.01
1a	2.8	6	1-2
1b	6	12	3
1c	5.7	11	2-3
2	4.2	8	2
3	12.2	24	6
4	7.7	15	3-4
5	7	14
6	15	30	7
7	5.6	11	3-4
8	12.2	24	6
9	1.4	3	1
10	8.4	17	4
11	6.6	13	3
12	5.2	10	2-3
total GCs		200	50
sACs	32.7	98	27
other dACs	3.9	12	3
Total dACs	3.9	110	30
Total Neurons		310	80

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