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Retinal Connectome Mosaics slice 001

RC1 0001_mosaic_8.png downsampled 8x to 118 Mb compressed

RC1 0001_mosaic_32.png downsampled 32x to 14 Mb compressed

Small 1024 x 1024 screen version of RC1 0001_mosaic Downsampled to 1.2 Mb compressed
Retinal Connectome dataset RC1 slice 001 visualized by TEM. This is a horizontal TEM plane through the retinal inner nuclear layer composed of over 1000 individual TEM images automatically captured as described in Anderson et al. 2009 (PLoS Biol 7, e1000074) on a JEOL JEM 1400 using a Gatan UltraScan 4000 camera. The center of a canonical field 243 μm in diameter was identified in the slot grid using SerialEM (D. N. Mastronarde 2006 , J Struct Biol 152: 32), and captured as an array of image tiles at 950-1100 tiles/section and 2.18 nm/pixel resolution. Each tile has 15% area overlap with its neighbors. Image mosaics and volumes were generated using the NCR Toolset (http://www.sci.utah.edu/download/ncrtoolset). SerialEM metadata position information used by the NCR Toolset application ir-translate produces precise initial image mosaics that are refined by ir-refine-grid to correct for image aberrations. Slice-to-slice TEM image registration is automated by ir-stos-brute, ir-stos-grid. CMP-to-TEM registrations are operator-guided with ir-tweak.


Retinal Connectome Mosaic slice 001 and registered CMP channels (TEM 32x downscaled, CMP upscaled)

0001_mosaic_32_complete_TEM.tif 11.9 Mb LZW

0001_mosaic_32_complete_channel_b.tif 10.1 Mb LZW

0001_mosaic_32_complete_channel_e.tif 9.5 Mb LZW

0001_mosaic_32_complete_channel_g.tif 6.2 Mb LZW

0001_mosaic_32_complete_channel_q.tif 11.4 Mb LZW

0001_mosaic_32_complete_channel_tt.tif 10.6 Mb LZW

0001_mosaic_32_complete_channel_yy.tif 1.1 Mb LZW


Retinal Connectome Mosaic slices and registered CMP channels (TEM 32x downscaled, CMP upscaled)

030_mosaic_32_complete_TEM.tif 12.7 Mb LZW

030_mosaic_32_complete_channel_g.tif 12.6 Mb LZW

062_mosaic_32_complete_TEM.tif 12.7 Mb LZW

062_mosaic_32_complete_channel_yy.tif 13.4 Mb LZW


Retinal Connectome Mosaics slice 030

RC1 0033_mosaic_8 downsampled 8x to 126 Mb compressed

RC1 0033_mosaic_32 sizedownsampled 32x to 16 Mb compressed

Small 1024 x 1024 screen version of RC1 0033 downsampled to 1.2 Mb compressed Retinal Connectome dataset RC1 slice 030 visualized by TEM.


Retinal Connectome Mosaics slice 0174 resolution series

RC1 00174 001 *.png Viking viewer capture at resolution 1 x 2.18 nm/pixel

RC1 00174 002 *.png Viking viewer capture at resolution 2 x 2.18 nm/pixel

RC1 00174 004 *.png Viking viewer capture at resolution 4 x 2.18 nm/pixel

RC1 00174 008 *.png Viking viewer capture at resolution 8 x 2.18 nm/pixel

RC1 00174 016 *.png Viking viewer capture at resolution 16 x 2.18 nm/pixel

RC1 00174 032 *.png Viking viewer capture at resolution 32 x 2.18 nm/pixel

RC1 00174 064 *.png Viking viewer capture at resolution 64 x 2.18 nm/pixel

RC1 00174 128 *.png Viking viewer capture at resolution 128 x 2.18 nm/pixel

Small RC1 00174 Quicktime ® Movie of step-through series Right-click or control-click to download 1 Mb.

Large 1024x768 RC1 00174 Quicktime ® Movie of step-through series Right-click or control-click to download 17 Mb.
A step-through resolution series on one slice deep in the Retinal Connectome dataset RC1 (slice 174) visualized by TEM. On resolutions 2 and 4, a small misregistration is marked with an arrow. This is the largest scale of misalignment produced by the relaxation algorithm




Owl Inferior Colliculus Mosaics

Owl 0014_mosaic_8.png downsampled 8x to 121 Mb compressed

Owl 0014_mosaic_32.png downsampled 32x to 9Mb compressed

Small 1024 x 1024 screen version of Owl 0014 mosaic downsampled to .8 Mb compressed


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