This is an animated gif of a series of transmission electron microscope images of the cilium of a photoreceptor. For this particular dataset, we tilted the grid on a single axis, in one direction to get a different look at the cilium when imaging, kind of like one might do with a CT scan to look at different angles. Theoretically, one could do computed tomography reconstruction of these sorts of data as well, tilting the grid in multiple axes to get a better look at the information, though that is not something we do. This particular section is not terribly thick, perhaps 90-100nm, but even so, there is depth information in the section that can be revealing.
By Bryan Jones — September 23, 2014 at 12:35 am